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antibodies against il1β  (R&D Systems)


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    R&D Systems antibodies against il1β
    TGFβ1 significantly reduces LPS-induced increases in NLRP3, CASP1, and <t>IL1β</t> protein levels in BV2 cells. After treatment with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and 12 h, protein levels were analyzed using western blotting. Representative western blot images for both experimental time points are depicted for NLRP3 (A) , CASP1 (C) , and IL1 β (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (B) , CASP1 (D) , and IL1β (F) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Antibodies Against Il1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 960 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+il1%CE%B2/Mouse+IL-1+beta%2FIL-1F2+Antibody/pmc12813031-71-6-10
    Average 97 stars, based on 960 article reviews
    antibodies against il1β - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming"

    Article Title: TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1623643

    TGFβ1 significantly reduces LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in BV2 cells. After treatment with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and 12 h, protein levels were analyzed using western blotting. Representative western blot images for both experimental time points are depicted for NLRP3 (A) , CASP1 (C) , and IL1 β (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (B) , CASP1 (D) , and IL1β (F) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: TGFβ1 significantly reduces LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in BV2 cells. After treatment with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and 12 h, protein levels were analyzed using western blotting. Representative western blot images for both experimental time points are depicted for NLRP3 (A) , CASP1 (C) , and IL1 β (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (B) , CASP1 (D) , and IL1β (F) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: Western Blot, Derivative Assay, Comparison

    TGFβ1 inhibits LPS-induced expression of Nlrp3 , Casp1 , Il18 , and Il1b in primary microglia. Microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and RNA and total proteins were isolated. TGFβ1 significantly inhibits LPS-induced upregulation of Nlrp3 (B) , Casp1 (B) , Il18 (C) , and Il1b (D) in primary microglia. TGFβ1 abrogates LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in microglia. Representative western blot images are depicted (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (F) , CASP1 (G) , and IL1β (H) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: TGFβ1 inhibits LPS-induced expression of Nlrp3 , Casp1 , Il18 , and Il1b in primary microglia. Microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and RNA and total proteins were isolated. TGFβ1 significantly inhibits LPS-induced upregulation of Nlrp3 (B) , Casp1 (B) , Il18 (C) , and Il1b (D) in primary microglia. TGFβ1 abrogates LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in microglia. Representative western blot images are depicted (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (F) , CASP1 (G) , and IL1β (H) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: Expressing, Isolation, Western Blot, Derivative Assay, Comparison

    TGFβ1 reduces release of inflammasome cleaved IL1β from primary microglia. Primary microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and inflammasome assembly was triggered using Nigericin (1.34mM/ul) treatment for additional 2 h. Proteins were isolated from whole cell lysates (WCL) and supernatants and pro- IL1β and cleaved IL1β was visualized. Representative western blot images are depicted (A) . Quantifications of pro-IL1β after normalization using β-Actin is presented (B) . Total levels of cleaved and bioactive IL1β in supernatants were quantified using ELISA. Quantifications of released IL1β are given (C) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are ** p < 0.01, and **** p < 0.0001.
    Figure Legend Snippet: TGFβ1 reduces release of inflammasome cleaved IL1β from primary microglia. Primary microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and inflammasome assembly was triggered using Nigericin (1.34mM/ul) treatment for additional 2 h. Proteins were isolated from whole cell lysates (WCL) and supernatants and pro- IL1β and cleaved IL1β was visualized. Representative western blot images are depicted (A) . Quantifications of pro-IL1β after normalization using β-Actin is presented (B) . Total levels of cleaved and bioactive IL1β in supernatants were quantified using ELISA. Quantifications of released IL1β are given (C) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are ** p < 0.01, and **** p < 0.0001.

    Techniques Used: Isolation, Western Blot, Enzyme-linked Immunosorbent Assay, Derivative Assay, Comparison

    Related Articles

    Western Blot:

    Article Title: The inhibitory innate immune sensor NLRP12 maintains a threshold against obesity by regulating gut microbiota homeostasis
    Article Snippet: F4/80 positive staining or liver steatosis area was quantified by ImageJ and displayed as % to total (stained + non-stained) area. .. For western blot analysis, the following primary antibodies were used for western blot analysis: anti-CASPASE1 (4D4) (Genentech); anti-IL1β (cat. AF-401-NA) (R&D systems); anti-pp65 (Ser536) (93H1) (cat. no. 3033); anti-p-ERK1/2 (Thr202/Tyr204) (D13.14.4E) (cat. no. 4370); anti-p65 (D14E12) (cat. no. 8242) (Cell signaling); antiREG3G (cat. no. ab198216, Abcam); anti-CRAMP (G1) (cat. no. sc-166055), anti-p52 (C-5) (cat. no. sc-7386); anti-ERK1 (C-16) (cat. no. sc-93); anti-ERK2 (C-14) (cat. no. sc-154) and anti-Actin-HRP (C-11) (cat. no. sc-1615) (Santa Cruz Biotechnology); and anti-Histone H3 (cat. no. 07-690) (Millipore). .. Goat anti-rabbit-IgG HRP (cat. no. 111035-144), goat anti-mouse-IgG HRP (cat. no. 115-035-146) and rabbit anti-goat-IgG HRP (cat no. 305-035-045) (Jackson Immune Research Laboratories) were used as secondary antibodies.

    Article Title: Golexanolone improves fatigue, motor incoordination and gait and memory in rats with bile duct ligation.
    Article Snippet: .. Protein content was analysed by Western blot as in.35 Primary antibodies used were antiTNFα (AF-510-NA), anti-IL1β (AF-501-NA) 1:250 and anti-IFN-γ (MAB5851) 1:1000 from R&D Systems; anti-IL-6 (ARC0062) 1:500 from BIOSOURCE; anti-IL-17 (ab79056), anti-IL-18 (ab19160), antiHMGB1 (ab18256) 1:1000 and anti- β-actin (ab6276-100) 1:5000 from ABCAM; anti-IL-15 (1829R) 1:2000 from BIOSS; anti-TGFβ (PA5-99186) 1:1000, anti-IL-10 (ab9969) and anti-CCL20 (AB9829) 1:1000 from ABCAM; anti-CCL5 (710001) 1:500 from Invitrogen and anti-CCL2 (66272-1-lg) 1:2000 from Proteintech. .. Secondary antibodies (1:4000) against rabbit, mouse, or goat were IgGs conjugated with alkaline phosphatase (Sigma).

    Article Title: High Glucose Aggravates Cerebral Ischemia/Reperfusion via Truncated NLRP3 ‐Mediated Hexokinase‐2 Translocation
    Article Snippet: .. The primary antibodies used in the present study were anti‐NLRP3 (#AG‐20B‐0014, AdipoGen, San Diego, CA, USA, 1:1000 for Western blotting, 2 μg/sample for immunoprecipitation), anti‐Casp‐1 (#Ag‐20B‐0042, AdipoGen, 1:1000), anti‐ASC (#67824, Cell Signaling Technology, Beverly, MA, USA, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐IL1β (#AF‐401‐NA; R&D Systems, 1:1000), anti‐Hexokinase (# EPR20839 , Abcam, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐TOM20 (#42406, Cell Signaling Technology, 1:1000 for Western blotting), anti‐GSDMD (#ab219800, Abcam, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐β‐actin (#P01L082, Gene‐Protein Link, 1:1000 for Western blotting), anti‐Flag tag (#F3165, Sigma‐Aldrich, 1:2000 for Western blotting), anti‐Iba1 (#N100‐1028, Novus Biologicals, 1:200 for immunofluorescence), anti‐Iba1 (#17198, Cell Signaling Technology, 1:1000 for Western blotting), anti‐HA tag (#AE008, Abclonal, 1:1000 for Western blotting), anti‐Myc tag (#E022050‐01, EARTH, 1:1000 for Western blotting), anti‐β‐tubulin (#CW0265, CWBIO, 1:1000 for Western blotting), anti‐PKA (#4782, CST, 1:1000 for Western blotting). .. BV2 microglial cells, immortalized bone marrow‐derived macrophages (iBMDMs) and HEK293T cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (#11965–092, Life Technologies) supplemented with 10% heat‐inactivated fetal bovine serum (FBS, #04–001‐1A, Biological Industries, Beit Haemek, Israel) and 1% penicillin–streptomycin solution (#03–031‐1B, Biological Industries) at 37°C in a humidified atmosphere with 5% CO 2 .

    Cell Based Assay:

    Article Title: Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Article Snippet: Phosphonoformic acid (PFA) (P6801, Sigma-Aldrich) and BAY 11-7082 (S2913, Selleckchem) were used as agents to elucidate underlying signal transduction mechanisms. .. Anti-IL6 (MP5-20F3, R&D Systems) and anti-IL1β (AF-401-NA, R&D Systems) were used as antibodies in a cell-based assay to neutralize the biological activity of targeted cytokines. ..

    Article Title: Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Article Snippet: PFA (P6801, Sigma- Aldrich) and BAY 11- 7082 (S2913, Selleckchem) were used as agents to elucidate underlying signal transduction mechanisms. .. Anti- IL6 (MP5- 20F3, R&D Systems) and anti- IL1β (AF- 401- NA, R&D Systems) were used as antibodies in a cell- based assay to neutralize the biological activity of targeted cytokines. ..

    Article Title: Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Article Snippet: PFA (P6801, Sigma-Aldrich) and BAY 11-7082 (S2913, Selleckchem) were used as agents to elucidate underlying signal transduction mechanisms. .. Anti-IL6 (MP5-20F3, R&D Systems) and anti-IL1β (AF-401-NA, R&D Systems) were used as antibodies in a cell-based assay to neutralize the biological activity of targeted cytokines. ..

    Activity Assay:

    Article Title: Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Article Snippet: Phosphonoformic acid (PFA) (P6801, Sigma-Aldrich) and BAY 11-7082 (S2913, Selleckchem) were used as agents to elucidate underlying signal transduction mechanisms. .. Anti-IL6 (MP5-20F3, R&D Systems) and anti-IL1β (AF-401-NA, R&D Systems) were used as antibodies in a cell-based assay to neutralize the biological activity of targeted cytokines. ..

    Article Title: Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Article Snippet: PFA (P6801, Sigma- Aldrich) and BAY 11- 7082 (S2913, Selleckchem) were used as agents to elucidate underlying signal transduction mechanisms. .. Anti- IL6 (MP5- 20F3, R&D Systems) and anti- IL1β (AF- 401- NA, R&D Systems) were used as antibodies in a cell- based assay to neutralize the biological activity of targeted cytokines. ..

    Article Title: Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Article Snippet: PFA (P6801, Sigma-Aldrich) and BAY 11-7082 (S2913, Selleckchem) were used as agents to elucidate underlying signal transduction mechanisms. .. Anti-IL6 (MP5-20F3, R&D Systems) and anti-IL1β (AF-401-NA, R&D Systems) were used as antibodies in a cell-based assay to neutralize the biological activity of targeted cytokines. ..

    Immunoprecipitation:

    Article Title: High Glucose Aggravates Cerebral Ischemia/Reperfusion via Truncated NLRP3 ‐Mediated Hexokinase‐2 Translocation
    Article Snippet: .. The primary antibodies used in the present study were anti‐NLRP3 (#AG‐20B‐0014, AdipoGen, San Diego, CA, USA, 1:1000 for Western blotting, 2 μg/sample for immunoprecipitation), anti‐Casp‐1 (#Ag‐20B‐0042, AdipoGen, 1:1000), anti‐ASC (#67824, Cell Signaling Technology, Beverly, MA, USA, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐IL1β (#AF‐401‐NA; R&D Systems, 1:1000), anti‐Hexokinase (# EPR20839 , Abcam, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐TOM20 (#42406, Cell Signaling Technology, 1:1000 for Western blotting), anti‐GSDMD (#ab219800, Abcam, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐β‐actin (#P01L082, Gene‐Protein Link, 1:1000 for Western blotting), anti‐Flag tag (#F3165, Sigma‐Aldrich, 1:2000 for Western blotting), anti‐Iba1 (#N100‐1028, Novus Biologicals, 1:200 for immunofluorescence), anti‐Iba1 (#17198, Cell Signaling Technology, 1:1000 for Western blotting), anti‐HA tag (#AE008, Abclonal, 1:1000 for Western blotting), anti‐Myc tag (#E022050‐01, EARTH, 1:1000 for Western blotting), anti‐β‐tubulin (#CW0265, CWBIO, 1:1000 for Western blotting), anti‐PKA (#4782, CST, 1:1000 for Western blotting). .. BV2 microglial cells, immortalized bone marrow‐derived macrophages (iBMDMs) and HEK293T cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (#11965–092, Life Technologies) supplemented with 10% heat‐inactivated fetal bovine serum (FBS, #04–001‐1A, Biological Industries, Beit Haemek, Israel) and 1% penicillin–streptomycin solution (#03–031‐1B, Biological Industries) at 37°C in a humidified atmosphere with 5% CO 2 .

    Immunofluorescence:

    Article Title: High Glucose Aggravates Cerebral Ischemia/Reperfusion via Truncated NLRP3 ‐Mediated Hexokinase‐2 Translocation
    Article Snippet: .. The primary antibodies used in the present study were anti‐NLRP3 (#AG‐20B‐0014, AdipoGen, San Diego, CA, USA, 1:1000 for Western blotting, 2 μg/sample for immunoprecipitation), anti‐Casp‐1 (#Ag‐20B‐0042, AdipoGen, 1:1000), anti‐ASC (#67824, Cell Signaling Technology, Beverly, MA, USA, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐IL1β (#AF‐401‐NA; R&D Systems, 1:1000), anti‐Hexokinase (# EPR20839 , Abcam, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐TOM20 (#42406, Cell Signaling Technology, 1:1000 for Western blotting), anti‐GSDMD (#ab219800, Abcam, 1:1000 for Western blotting, 1:200 for immunofluorescence), anti‐β‐actin (#P01L082, Gene‐Protein Link, 1:1000 for Western blotting), anti‐Flag tag (#F3165, Sigma‐Aldrich, 1:2000 for Western blotting), anti‐Iba1 (#N100‐1028, Novus Biologicals, 1:200 for immunofluorescence), anti‐Iba1 (#17198, Cell Signaling Technology, 1:1000 for Western blotting), anti‐HA tag (#AE008, Abclonal, 1:1000 for Western blotting), anti‐Myc tag (#E022050‐01, EARTH, 1:1000 for Western blotting), anti‐β‐tubulin (#CW0265, CWBIO, 1:1000 for Western blotting), anti‐PKA (#4782, CST, 1:1000 for Western blotting). .. BV2 microglial cells, immortalized bone marrow‐derived macrophages (iBMDMs) and HEK293T cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (#11965–092, Life Technologies) supplemented with 10% heat‐inactivated fetal bovine serum (FBS, #04–001‐1A, Biological Industries, Beit Haemek, Israel) and 1% penicillin–streptomycin solution (#03–031‐1B, Biological Industries) at 37°C in a humidified atmosphere with 5% CO 2 .



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    TGFβ1 significantly reduces LPS-induced increases in NLRP3, CASP1, and <t>IL1β</t> protein levels in BV2 cells. After treatment with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and 12 h, protein levels were analyzed using western blotting. Representative western blot images for both experimental time points are depicted for NLRP3 (A) , CASP1 (C) , and IL1 β (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (B) , CASP1 (D) , and IL1β (F) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
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    Image Search Results


    TGFβ1 significantly reduces LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in BV2 cells. After treatment with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and 12 h, protein levels were analyzed using western blotting. Representative western blot images for both experimental time points are depicted for NLRP3 (A) , CASP1 (C) , and IL1 β (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (B) , CASP1 (D) , and IL1β (F) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming

    doi: 10.3389/fimmu.2025.1623643

    Figure Lengend Snippet: TGFβ1 significantly reduces LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in BV2 cells. After treatment with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and 12 h, protein levels were analyzed using western blotting. Representative western blot images for both experimental time points are depicted for NLRP3 (A) , CASP1 (C) , and IL1 β (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (B) , CASP1 (D) , and IL1β (F) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: They were later incubated with primary antibodies against Il1β (AF-401-NA, R&D Systems, 1:500), Nlrp3 (AG-20B-0014, Adipogen, 1:500), Caspase-1 (AG-20B-0042, Adipogen, 1:500) and β-Actin (#4967, Cell Signaling,1:2000) overnight at 4 °C.

    Techniques: Western Blot, Derivative Assay, Comparison

    TGFβ1 inhibits LPS-induced expression of Nlrp3 , Casp1 , Il18 , and Il1b in primary microglia. Microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and RNA and total proteins were isolated. TGFβ1 significantly inhibits LPS-induced upregulation of Nlrp3 (B) , Casp1 (B) , Il18 (C) , and Il1b (D) in primary microglia. TGFβ1 abrogates LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in microglia. Representative western blot images are depicted (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (F) , CASP1 (G) , and IL1β (H) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming

    doi: 10.3389/fimmu.2025.1623643

    Figure Lengend Snippet: TGFβ1 inhibits LPS-induced expression of Nlrp3 , Casp1 , Il18 , and Il1b in primary microglia. Microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and RNA and total proteins were isolated. TGFβ1 significantly inhibits LPS-induced upregulation of Nlrp3 (B) , Casp1 (B) , Il18 (C) , and Il1b (D) in primary microglia. TGFβ1 abrogates LPS-induced increases in NLRP3, CASP1, and IL1β protein levels in microglia. Representative western blot images are depicted (E) . Quantifications after normalization using β-Actin are presented for NLRP3 (F) , CASP1 (G) , and IL1β (H) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: They were later incubated with primary antibodies against Il1β (AF-401-NA, R&D Systems, 1:500), Nlrp3 (AG-20B-0014, Adipogen, 1:500), Caspase-1 (AG-20B-0042, Adipogen, 1:500) and β-Actin (#4967, Cell Signaling,1:2000) overnight at 4 °C.

    Techniques: Expressing, Isolation, Western Blot, Derivative Assay, Comparison

    TGFβ1 reduces release of inflammasome cleaved IL1β from primary microglia. Primary microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and inflammasome assembly was triggered using Nigericin (1.34mM/ul) treatment for additional 2 h. Proteins were isolated from whole cell lysates (WCL) and supernatants and pro- IL1β and cleaved IL1β was visualized. Representative western blot images are depicted (A) . Quantifications of pro-IL1β after normalization using β-Actin is presented (B) . Total levels of cleaved and bioactive IL1β in supernatants were quantified using ELISA. Quantifications of released IL1β are given (C) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are ** p < 0.01, and **** p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: TGFβ1 attenuates microglial IL1β release through inhibition of NLRP3 inflammasome priming

    doi: 10.3389/fimmu.2025.1623643

    Figure Lengend Snippet: TGFβ1 reduces release of inflammasome cleaved IL1β from primary microglia. Primary microglia were treated with TGFβ1 (5 ng/ml), LPS (1 µg/ml) or the combination of both factors for 6 h and inflammasome assembly was triggered using Nigericin (1.34mM/ul) treatment for additional 2 h. Proteins were isolated from whole cell lysates (WCL) and supernatants and pro- IL1β and cleaved IL1β was visualized. Representative western blot images are depicted (A) . Quantifications of pro-IL1β after normalization using β-Actin is presented (B) . Total levels of cleaved and bioactive IL1β in supernatants were quantified using ELISA. Quantifications of released IL1β are given (C) . Data are given as means ± SEM from at least three independent experiments. P-values derived from one-way ANOVA followed by Tukey´s multiple comparison tests are ** p < 0.01, and **** p < 0.0001.

    Article Snippet: They were later incubated with primary antibodies against Il1β (AF-401-NA, R&D Systems, 1:500), Nlrp3 (AG-20B-0014, Adipogen, 1:500), Caspase-1 (AG-20B-0042, Adipogen, 1:500) and β-Actin (#4967, Cell Signaling,1:2000) overnight at 4 °C.

    Techniques: Isolation, Western Blot, Enzyme-linked Immunosorbent Assay, Derivative Assay, Comparison